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human ip 10 precoated elisa kit  (Dakewe Biotech Co)

 
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    Dakewe Biotech Co human ip 10 precoated elisa kit
    Human Ip 10 Precoated Elisa Kit, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ip+10+precoated+elisa+kit/10+elisa+human+ip+kit+precoated/pmc13006404-375-5-10
    Average 86 stars, based on 1 article reviews
    human ip 10 precoated elisa kit - by Bioz Stars, 2026-10
    86/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Identification and characterization of bone marrow plasma cells producing IFN-γ-neutralizing autoantibodies in adult-onset immunodeficiency
    Article Snippet: CD138 MicroBeads , Miltenyi , Cat# 130-097-614. .. Human IP-10 Precoated ELISA Kit , Dakewe , Cat# 1117452. .. SeekOne DD Single Cell 5′ Library Preparation Kit , SeekGene , Cat# K00501.

    Article Title: Identification and characterization of bone marrow plasma cells producing IFN-γ-neutralizing autoantibodies in adult-onset immunodeficiency
    Article Snippet: Following surface staining, cells were then fixed and permeabilized with 100 μL Cytofix/Cytoperm Fixation/Permeabilization Solution for 20 min. Intracellular staining was performed using CoraLite647-conjugated iNOS (1:100) in 50 μL of staining buffer at 4°C for 20 min. Data acquisition was conducted using an LSRFortessa flow cytometer (gating strategy shown in A), and analysis was performed using FlowJo software to calculate the fold change in median fluorescence intensity (MFI) of HLA-ABC, HLA-DR, CD80, and iNOS relative to the unstimulated control. .. CXCL10 concentration was measured using Human IP-10 Precoated ELISA Kit (Dakewe). ..

    Concentration Assay:

    Article Title: Identification and characterization of bone marrow plasma cells producing IFN-γ-neutralizing autoantibodies in adult-onset immunodeficiency
    Article Snippet: Following surface staining, cells were then fixed and permeabilized with 100 μL Cytofix/Cytoperm Fixation/Permeabilization Solution for 20 min. Intracellular staining was performed using CoraLite647-conjugated iNOS (1:100) in 50 μL of staining buffer at 4°C for 20 min. Data acquisition was conducted using an LSRFortessa flow cytometer (gating strategy shown in A), and analysis was performed using FlowJo software to calculate the fold change in median fluorescence intensity (MFI) of HLA-ABC, HLA-DR, CD80, and iNOS relative to the unstimulated control. .. CXCL10 concentration was measured using Human IP-10 Precoated ELISA Kit (Dakewe). ..



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    human ip 10 precoated elisa kit  (Dakewe Biotech Co)
    86
    Dakewe Biotech Co human ip 10 precoated elisa kit
    Human Ip 10 Precoated Elisa Kit, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ip+10+precoated+elisa+kit/10+elisa+human+ip+kit+precoated/pmc13006404-375-5-10
    Average 86 stars, based on 1 article reviews
    human ip 10 precoated elisa kit - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    human ip-10 precoated elisa kit (cat#:1117452)  (Dakewe Biotech Co)
    90
    Dakewe Biotech Co human ip-10 precoated elisa kit (cat#:1117452)
    TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion of IFN-α and <t>CXCL10</t> were assayed by ELISA. These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.
    Human Ip 10 Precoated Elisa Kit (Cat#:1117452), supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ip+10+precoated+elisa+kit/human+ip+10+precoated+elisa+kit++cat++1117452+/pmc11061751-329-12-5
    Average 90 stars, based on 1 article reviews
    human ip-10 precoated elisa kit (cat#:1117452) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    human ip-10 precoated elisa kit  (Dakewe Biotech Co)
    90
    Dakewe Biotech Co human ip-10 precoated elisa kit
    TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion <t>of</t> <t>IFN-α</t> and CXCL10 were assayed by <t>ELISA.</t> These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.
    Human Ip 10 Precoated Elisa Kit, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ip+10+precoated+elisa+kit/human+ip+10+precoated+elisa+kit++cat++1117452+/pmc11061751-329-15-5
    Average 90 stars, based on 1 article reviews
    human ip-10 precoated elisa kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion of IFN-α and CXCL10 were assayed by ELISA. These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.

    Journal: The Journal of Biological Chemistry

    Article Title: Epstein-Barr virus suppresses N 6 -methyladenosine modification of TLR9 to promote immune evasion

    doi: 10.1016/j.jbc.2024.107226

    Figure Lengend Snippet: TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion of IFN-α and CXCL10 were assayed by ELISA. These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.

    Article Snippet: ELISA kits were obtained from Dakewe: Human IFN-α Precoated ELISA Kit (Cat#:1110012), Human IP-10 Precoated ELISA Kit (Cat#:1117452), Human IgA Precoated ELISA Kit (Cat#:1128172), Human IgM Precoated ELISA Kit (Cat#:1128182), Human IgG Precoated ELISA Kit(Cat#:1128162), Human IL-6 Precoated ELISA Kit(Cat#:1110602), Human IL-8 Precoated ELISA Kit (Cat#:1110802).

    Techniques: Expressing, Modification, Dot Blot, Western Blot, Quantitative RT-PCR, Isolation, Infection, Enzyme-linked Immunosorbent Assay, Virus, Real-time Polymerase Chain Reaction

    TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion of IFN-α and CXCL10 were assayed by ELISA. These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.

    Journal: The Journal of Biological Chemistry

    Article Title: Epstein-Barr virus suppresses N 6 -methyladenosine modification of TLR9 to promote immune evasion

    doi: 10.1016/j.jbc.2024.107226

    Figure Lengend Snippet: TLR9 downstream signaling pathways and cytokine expression are regulated by METTL3. A , cellular m 6 A modification levels were assayed in BJAB-shNC and BJAB-shMETTL3 cells by Dot blot analysis. B , the expression levels of TLR9 and METTL3 were determined by Western blotting in BJAB-shNC and BJAB-shMETTL3 cells. C , BJAB-shNC and BJAB-shMETTL3 cells were treated with ActD (5 μg/ml) for 0, 3, and 6 h; TLR9 mRNA levels were measured by RT-qPCR. D , the indicated TLR9 signaling proteins were detected by cytoplasmic and nuclear proteins isolation. GAPDH and Histone H3 were used for cytoplasm and nucleus protein loading controls, respectively. E , BJAB cells were treated with STM2457(10 μM) and CpG-ODN2006 (5 μM) upon 50 MOI EBV infection, respectively. EBV genomes copy number was determined by qPCR. F , primary B cells were treated with STM2457(10 μM) and CpG-ODN2006(5 μM) with or without 50MOI EBV infection for appointed time. The secretion of IFN-α and CXCL10 were assayed by ELISA. These data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. EBNA1, Epstein-Barr nuclear antigen 1; EBV, Epstein-Barr virus; IFN, interferon; m 6 A, N 6 -methyladenosine; MOI, multiplicity of infection; ODN, oligodeoxynucleotide; RT-qPCR, reverse transcription-quantitative PCR; TLR9, toll-like receptor 9.

    Article Snippet: ELISA kits were obtained from Dakewe: Human IFN-α Precoated ELISA Kit (Cat#:1110012), Human IP-10 Precoated ELISA Kit (Cat#:1117452), Human IgA Precoated ELISA Kit (Cat#:1128172), Human IgM Precoated ELISA Kit (Cat#:1128182), Human IgG Precoated ELISA Kit(Cat#:1128162), Human IL-6 Precoated ELISA Kit(Cat#:1110602), Human IL-8 Precoated ELISA Kit (Cat#:1110802).

    Techniques: Protein-Protein interactions, Expressing, Modification, Dot Blot, Western Blot, Quantitative RT-PCR, Isolation, Infection, Enzyme-linked Immunosorbent Assay, Virus, Reverse Transcription, Real-time Polymerase Chain Reaction

    STM2457 inhibits CpG-ODN-induced B cell proliferation, Ig secretion and cytokine expressions. A , BJAB cells were treated with STM2457 for 96 h. Cellular m 6 A modification levels were assayed by dot blot analysis. TLR9 signaling molecules were assayed by Western blotting. B , gene set difference between the TLR9 high and low human immune cells was revealed by GSEA. The top gene set enriched in TLR9 high group comparing to low group is listed. C–E , PBMCs from healthy donors were stained with CFSE dyes and cultured with anti-IgM (10 mM), CD40L (1 mM), and IL-4 (10 nM), costimulated with STM2457(10 μM) or ODN-2006(5 μM) for 0 and 6 days. DMSO was used as a control. Only CD19+ B cells stained CFSE dyes were gated by flow cytometry analysis ( C and D ). E , histogram shows the percentage of B cells dividing under different conditions. F and G , fresh PBMCs cultured with anti-IgM, CD40L, IL-4, and costimulated with STM2457(10 μM) or ODN-2006(5 μM) for 8 days were collected to detect Ig-producing cells ( F ) and cytokine level in supernatants by ELISA analysis ( G ). H and I , BJAB-shRNA cells were transfected with HA-TLR9 plasmids for 48 h. Then, the levels of Ig ( H ) and cytokine ( I ) in supernatants were detected by ELISA analysis. J , model diagram demonstrates the role of STM2457 in the regulation of B-cell proliferation and cytokines secretion. Experiments were independently repeated three times, and results are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant, compared with the control group. CFSE, carboxyfluorescein succinimidyl ester; DMSO, dimethylsulfoxide; GSEA, gene set enrichment analysis; Ig, immunoglobulin; IL, interleukin; m 6 A, N 6 -methyladenosine; ODN, oligodeoxynucleotide; PBMC, peripheral blood mononuclear cell; TLR9, toll-like receptor 9.

    Journal: The Journal of Biological Chemistry

    Article Title: Epstein-Barr virus suppresses N 6 -methyladenosine modification of TLR9 to promote immune evasion

    doi: 10.1016/j.jbc.2024.107226

    Figure Lengend Snippet: STM2457 inhibits CpG-ODN-induced B cell proliferation, Ig secretion and cytokine expressions. A , BJAB cells were treated with STM2457 for 96 h. Cellular m 6 A modification levels were assayed by dot blot analysis. TLR9 signaling molecules were assayed by Western blotting. B , gene set difference between the TLR9 high and low human immune cells was revealed by GSEA. The top gene set enriched in TLR9 high group comparing to low group is listed. C–E , PBMCs from healthy donors were stained with CFSE dyes and cultured with anti-IgM (10 mM), CD40L (1 mM), and IL-4 (10 nM), costimulated with STM2457(10 μM) or ODN-2006(5 μM) for 0 and 6 days. DMSO was used as a control. Only CD19+ B cells stained CFSE dyes were gated by flow cytometry analysis ( C and D ). E , histogram shows the percentage of B cells dividing under different conditions. F and G , fresh PBMCs cultured with anti-IgM, CD40L, IL-4, and costimulated with STM2457(10 μM) or ODN-2006(5 μM) for 8 days were collected to detect Ig-producing cells ( F ) and cytokine level in supernatants by ELISA analysis ( G ). H and I , BJAB-shRNA cells were transfected with HA-TLR9 plasmids for 48 h. Then, the levels of Ig ( H ) and cytokine ( I ) in supernatants were detected by ELISA analysis. J , model diagram demonstrates the role of STM2457 in the regulation of B-cell proliferation and cytokines secretion. Experiments were independently repeated three times, and results are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant, compared with the control group. CFSE, carboxyfluorescein succinimidyl ester; DMSO, dimethylsulfoxide; GSEA, gene set enrichment analysis; Ig, immunoglobulin; IL, interleukin; m 6 A, N 6 -methyladenosine; ODN, oligodeoxynucleotide; PBMC, peripheral blood mononuclear cell; TLR9, toll-like receptor 9.

    Article Snippet: ELISA kits were obtained from Dakewe: Human IFN-α Precoated ELISA Kit (Cat#:1110012), Human IP-10 Precoated ELISA Kit (Cat#:1117452), Human IgA Precoated ELISA Kit (Cat#:1128172), Human IgM Precoated ELISA Kit (Cat#:1128182), Human IgG Precoated ELISA Kit(Cat#:1128162), Human IL-6 Precoated ELISA Kit(Cat#:1110602), Human IL-8 Precoated ELISA Kit (Cat#:1110802).

    Techniques: Modification, Dot Blot, Western Blot, Staining, Cell Culture, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay, shRNA, Transfection